Pengujian Aktivitas Fungsional Makrofag Alveolar Post Diinduksi Asap Rokok

Abstract

Background: The increasing incidence of smoking causes an increase in the death rate due to smoking. Cigarette smoke can affect alveolar macrophages as part of the nonspecific immune system. The study was to see the effect of secondhand smoke exposure on the decrease in functional activity of alveolar macrophages. Methods: This was an experimental study using a post-test only design, conducted at the Laboratory of Microbiology and Biomedical Faculty of Medicine Andalas University. For the in vitro study, Wistar rats were exposed to cigarette smoke for three weeks; a control group was maintained. Macrophages were isolated from the lungs of both groups, cultured, and tested for bactericidal activity against Klebsiella pneumoniae. For the in vivo study, similar groups of Wistar rats were used. Both groups received intrapulmonary injections of Escherichia coli for three days. Lung suspensions were cultured, and bacterial colonies were counted. Results: By unpaired t-test, in vitro studies show a significant decline in macrophage bactericidal activity (P=0.030), whereas in vivo studies showed a decline in protective function against bacterial infection, but this was not significant (P=0.796). Conclusion: There is a decrease in the alveolar macrophages' ability induced by cigarette smoke.